Development of a method to produce hemoglobin in a bioreactor culture of Escherichia coli BL21(DE3) transformed with a plasmid containing Plesiomonas shigelloides heme transport genes and modified human hemoglobin genes.
نویسندگان
چکیده
We describe a method for production of recombinant human hemoglobin by Escherichia coli grown in a bioreactor. E. coli BL21(DE3) transformed with a plasmid containing hemoglobin genes and Plesiomonas shigelloides heme transport genes reached a cell dry weight of 83.64 g/liter and produced 11.92 g/liter of hemoglobin in clarified lysates.
منابع مشابه
Iron uptake in Plesiomonas shigelloides: cloning of the genes for the heme-iron uptake system.
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 77 18 شماره
صفحات -
تاریخ انتشار 2011